Neutral red: A pH indicator that acts as a litmus paper. Gram negative species that live in the colon can resist bile salt's negative effects.Ĭrystal violet: Inhibits the growth of gram positive bacteria by inducing DNA lesions. Peptone: Nitrogen source, also works as a carbon source for bacteria that can not metabolize sugars.īile salts: Inhibit the growth of gram positive bacteria by disrupting the membranes. To understand how MacConkey agar works, let's first see the role of different ingredients.
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